Journal: ACS Infectious Diseases

Article Title: Diaryl-Aminoindazole with Extensive In Vitro Mycobactericidal Activity Dependent on Exposure to Reactive Nitrogen Species

doi: 10.1021/acsinfecdis.5c00879

Figure Lengend Snippet: RNS-dependent activity of GSK1867513A against nontuberculous mycobacteria M. avium and M. abscessus . (A) Concentration-dependent mycobactericidal activity of GSK513A against M. avium subspecies hominissuis MAC101 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in one experiment. (B) Concentration-dependent mycobacteristatic activity of GSK513A against M. abscessus subspecies abscessus ATCC 19977 in the presence of DETANO, but not DETA, at pH 6.8. Means ± SD of triplicates in two experiments.

Article Snippet: Mtb H37Rv, M. abscessus subsp. abscessus ATCC 19977 (Mabs) and M. avium subsp. hominissuis MAC101 (MAC) were cultured in 7H9 medium with 0.2% glycerol, 0.02% tyloxapol and 10% OADC (Difco) (complete 7H9).

Techniques: Activity Assay, Concentration Assay

Journal: Journal of Clinical Microbiology

Article Title: Development of a Rapid ATP Bioluminescence Assay for Biocidal Susceptibility Testing of Rapidly Growing Mycobacteria ▿

doi: 10.1128/JCM.01482-10

Figure Lengend Snippet: Mycobactericidal activity of Cidexplus as measured in a cell suspension assay format using the agar plate count method and the developed ATP bioluminescence assay

Article Snippet: The mycobacterial cultures were grown in Middlebrook 7H9 (MB7H9) broth, supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC) enrichment (BD Biosciences, Sparks, MD) and 0.05% Tween 80 as described previously ( 30 ) or Sauton's broth medium ( 28 ). table ft1 table-wrap mode="anchored" t5 caption a7 Mycobacterium species Cidexplus concn a (% [vol/vol]) Log initial count (log CFU) Log CFU reduction factor % reduction in RLU b 10 min 20 min 30 min 10 min 20 min 30 min M. chelonae ATCC 35752 1 6.4 6.4 6.4 6.4 100 100 100 2 6.4 6.4 6.4 100 100 100 M. abscessus ATCC 19977 1 7.2 1.5 1.72 2.8 88.3 99.03 100 2 7.2 7.2 7.2 100 100 100 M. immunogenum ATCC 700506 1 6.3 0.58 1.8 2.23 97.15 99 100 2 6.3 6.3 6.3 100 100 100 Open in a separate window a The test concentrations (1% and 2%) were selected based on the fact that 2% is the maximum concentration limit suggested for hospital disinfection applications. b The values represent % reduction in the normalized value (compared to the untreated control); normalization was done by subtracting the residual ATP value.

Techniques: Activity Assay, Suspension

Journal: Journal of Clinical Microbiology

Article Title: Development of a Rapid ATP Bioluminescence Assay for Biocidal Susceptibility Testing of Rapidly Growing Mycobacteria ▿

doi: 10.1128/JCM.01482-10

Figure Lengend Snippet: Sensitivities and specificities of the developed ATP bioluminescence assay for determining the MIC of Kathon

Article Snippet: The mycobacterial cultures were grown in Middlebrook 7H9 (MB7H9) broth, supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC) enrichment (BD Biosciences, Sparks, MD) and 0.05% Tween 80 as described previously ( 30 ) or Sauton's broth medium ( 28 ). table ft1 table-wrap mode="anchored" t5 caption a7 Mycobacterium species Cidexplus concn a (% [vol/vol]) Log initial count (log CFU) Log CFU reduction factor % reduction in RLU b 10 min 20 min 30 min 10 min 20 min 30 min M. chelonae ATCC 35752 1 6.4 6.4 6.4 6.4 100 100 100 2 6.4 6.4 6.4 100 100 100 M. abscessus ATCC 19977 1 7.2 1.5 1.72 2.8 88.3 99.03 100 2 7.2 7.2 7.2 100 100 100 M. immunogenum ATCC 700506 1 6.3 0.58 1.8 2.23 97.15 99 100 2 6.3 6.3 6.3 100 100 100 Open in a separate window a The test concentrations (1% and 2%) were selected based on the fact that 2% is the maximum concentration limit suggested for hospital disinfection applications. b The values represent % reduction in the normalized value (compared to the untreated control); normalization was done by subtracting the residual ATP value.

Techniques: ATP Bioluminescent Assay

Journal: Journal of Clinical Microbiology

Article Title: Development of a Rapid ATP Bioluminescence Assay for Biocidal Susceptibility Testing of Rapidly Growing Mycobacteria ▿

doi: 10.1128/JCM.01482-10

Figure Lengend Snippet: Comparison of different cell lysis protocols for ATP extraction from M. immunogenum ATCC 700506 (∼106 cells). Values presented are means ± standard deviations (n = 3). ATP release using lysozyme treatment was significantly improved (P value < 0.05) when combined with bead beating.

Article Snippet: The mycobacterial cultures were grown in Middlebrook 7H9 (MB7H9) broth, supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC) enrichment (BD Biosciences, Sparks, MD) and 0.05% Tween 80 as described previously ( 30 ) or Sauton's broth medium ( 28 ). table ft1 table-wrap mode="anchored" t5 caption a7 Mycobacterium species Cidexplus concn a (% [vol/vol]) Log initial count (log CFU) Log CFU reduction factor % reduction in RLU b 10 min 20 min 30 min 10 min 20 min 30 min M. chelonae ATCC 35752 1 6.4 6.4 6.4 6.4 100 100 100 2 6.4 6.4 6.4 100 100 100 M. abscessus ATCC 19977 1 7.2 1.5 1.72 2.8 88.3 99.03 100 2 7.2 7.2 7.2 100 100 100 M. immunogenum ATCC 700506 1 6.3 0.58 1.8 2.23 97.15 99 100 2 6.3 6.3 6.3 100 100 100 Open in a separate window a The test concentrations (1% and 2%) were selected based on the fact that 2% is the maximum concentration limit suggested for hospital disinfection applications. b The values represent % reduction in the normalized value (compared to the untreated control); normalization was done by subtracting the residual ATP value.

Techniques: Comparison, Lysis, Extraction

Journal: Journal of Clinical Microbiology

Article Title: Development of a Rapid ATP Bioluminescence Assay for Biocidal Susceptibility Testing of Rapidly Growing Mycobacteria ▿

doi: 10.1128/JCM.01482-10

Figure Lengend Snippet: Optimization of the inoculum size for Kathon susceptibility testing of M. immunogenum. (A) Small inoculum (104 CFU/ml). (B) Large inoculum (106 CFU/ml). Sauton's medium was used for culturing the test strain (M. immunogenum ATCC 700506).

Article Snippet: The mycobacterial cultures were grown in Middlebrook 7H9 (MB7H9) broth, supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC) enrichment (BD Biosciences, Sparks, MD) and 0.05% Tween 80 as described previously ( 30 ) or Sauton's broth medium ( 28 ). table ft1 table-wrap mode="anchored" t5 caption a7 Mycobacterium species Cidexplus concn a (% [vol/vol]) Log initial count (log CFU) Log CFU reduction factor % reduction in RLU b 10 min 20 min 30 min 10 min 20 min 30 min M. chelonae ATCC 35752 1 6.4 6.4 6.4 6.4 100 100 100 2 6.4 6.4 6.4 100 100 100 M. abscessus ATCC 19977 1 7.2 1.5 1.72 2.8 88.3 99.03 100 2 7.2 7.2 7.2 100 100 100 M. immunogenum ATCC 700506 1 6.3 0.58 1.8 2.23 97.15 99 100 2 6.3 6.3 6.3 100 100 100 Open in a separate window a The test concentrations (1% and 2%) were selected based on the fact that 2% is the maximum concentration limit suggested for hospital disinfection applications. b The values represent % reduction in the normalized value (compared to the untreated control); normalization was done by subtracting the residual ATP value.

Techniques: